Renoprotective Effect and Mechanism of Qizhi Zhenwu Decoction in Murine Models of Doca-Salt Hypertension
DOI: 10.23977/medsc.2023.040320 | Downloads: 8 | Views: 130
Chengcheng Ma 1, Qi Zhang 1, Hong Li 1, Zhaowen Li 1, Yingying Tan 1
1 Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, 712000 China
Corresponding AuthorYingying Tan
The purpose of this study was to evaluate the renal protective effect of Qizhi Zhenwu Decoction on rats with DOCA salt hypertension and its influencing mechanism. In this study, the left kidney of rats was excised, and deoxycorticosterone acetate (DOCA) was injected under the epidermis, and a rat model of DOCA salt hypertension was made. They were then randomly divided into five groups: sham group, model group, Spironolactone group (Spi), QZZWD low-dose group (QZL), and QZZWD high-dose group (QZH), each group containing 7 rats. Several physiological measurements were done as follows: After two weeks of drug intervention, the blood pressure of mice was measured by tail sleeve method. Reactive oxygen species (ROS) were determined by fluorescence staining. Blood urea nitrogen and creatinine were detected by intelligent biochemical analyzer. Urine protein was detected by biochemical kit; The gene expression levels of NOX2, p47phox, NF-KB-p65, TGF-, TNF- and IL-6 were detected by western blotting. Compared with the sham operation group, the DOCA salt group showed obvious advantages compared with the model reference group in the gene expression levels of urinary protein, constriction pressure, urea nitrogen, creatinine, ROS and TGF-β, NOX2, P-NF-KB-P65, p47phox, TNF-αand IL-6 (p<0.05). QZZWD may maintain renal function by restricting NOX2/ROS/NF-KB signaling channels.
KEYWORDSQizhi zhenwu decoction, Doca-salt hypertension, Kidney functions
CITE THIS PAPER
Chengcheng Ma, Qi Zhang, Hong Li, Zhaowen Li, Yingying Tan, Renoprotective Effect and Mechanism of Qizhi Zhenwu Decoction in Murine Models of Doca-Salt Hypertension. MEDS Clinical Medicine (2023) Vol. 4: 143-149. DOI: http://dx.doi.org/10.23977/medsc.2023.040320.
 Mennuni S, Rubattu S, Pierelli G, Tocci G, Fofi C, Volpe M. Hypertension and kidneys: unraveling complex molecular mechanisms underlying hypertensive renal damage. J Hum Hypertens. 28: 74-79. 2014.
 Mou Yongcheng, Ding Qiuhui, Zhang Weihua. Analysis of the effective rate and improvement of renal function in patients with renal hypertension treated with modified Zhenwu Decoction. Research on Chinese and foreign women's health. 2021: 2. 2021.
 Senoner T, Dichtl W. Oxidative Stress in Cardiovascular Diseases: Still a Therapeutic Target? Nutrients. 11: 2090. 2019.
 Chen Y, Feng X, Hu X, Sha J, Li B, Zhang H, Fan H. Dexmedetomidine Ameliorates Acute Stress-Induced Kidney Injury by Attenuating Oxidative Stress and Apoptosis through Inhibition of the ROS/JNK Signaling Pathway. Oxid Med Cell Longey. 2018: 4035310. 2018.
 Fishel Bartal M, Lindheimer MD, Sibai BM. Proteinuria during pregnancy: definition, pathophysiology, methodology, and clinical significance. Am J Obstet Gynecol. 226: S819-S834. 2022.
 Jaquet V, Scapozza L, Clark RA, Krause KH, Lambeth JD. Small-molecule NOX inhibitors: ROS-generating NADPH oxidases as therapeutic targets. Antioxid Redox Signal. 11: 2535-2552. 2009.
 Honda H, Nagai Y, Matsunaga T, Matsunaga T, Okamoto N, Watanabe Y, Tsuneyama K, Hayashi H, Fujii I, Ikutani M, Hiral Y, Muraguchi A, Takatsu K. Isoliquiritigenin is a potent inhibitor of NLRP3 inflammasome activation and diet-induced adipose tissue inflammation. J Leukoc Biol. 96: 1087-1100. 2014.