Education, Science, Technology, Innovation and Life
Open Access
Sign In

Screening and Characterization of Nitrogen Removal Functional Bacteria in Landfill Leachate

Download as PDF

DOI: 10.23977/erej.2023.070302 | Downloads: 3 | Views: 149


Chao Li 1, Zhengyang An 1, Chang Gao 1, Teng Cai 1, Shuying Huang 1, Xin Jin 1


1 College of Biological and Agricultural Sciences, Honghe University, Mengzi, Yunnan, 661199, China

Corresponding Author

Zhengyang An


In this paper, the microorganisms collected from the leachate of the landfill drainage outlet of the landfill were separated and purified for many times, and the sterilized and diluted leachate was added and physicochemical treatment as a blank control, and the ammonia nitrogen degradation rate was used as the evaluation index to measure the effect of purified and domesticated strains in treating leachate. After cultivation and screening, the final ammonia nitrogen degradation rates of different strains 3, 4, 7, 9, 10, 12 and 13 were 57.14%, 38.96%, 85.26%, 92.72%, 66.53%, 95.62% and 74.84%, the degradation rate of No. 12 strain was as high as 95.62%, and the lowest degradation rate was 38.96% for No. 4 strain. The research showed that the degradation effects of different strains were different, and strains with good degradation of ammonia nitrogen were screened out. In summary, the microorganisms isolated from the landfill leachate can degrade the ammonia nitrogen present in themselves, thereby reducing the pollution of its water quality, and indirectly or directly affecting the effect of environmental indicators.


Ammonia nitrogen degradation rate, microbe, landfill leachate, separation and purification, evolutionary tree


Chao Li, Zhengyang An, Chang Gao, Teng Cai, Shuying Huang, Xin Jin, Screening and Characterization of Nitrogen Removal Functional Bacteria in Landfill Leachate. Environment, Resource and Ecology Journal (2023) Vol. 7: 9-20. DOI:


[1] Lan Mu. Analysis of the current situation and existing problems of landfill leachate treatment. Resource Economization & environmental protection. 2022 (12): 115 – 118 
[2] Qian Xu, Ting Ling. Middle-aged and aged landfill leachate treatment technology and related research progress. Science & Technology Vision, 2021 (27): 192 -193. TWO: 10. 19319694/ j. cnki. issn 2095 – 2457. 2021. 27. 80.
[3] Lina Wu, Zhi Li, Mingyu Shen; New treatment technology and application of landfill leachate, Beijing: Chemical Industry Press, 2019: 1-2. 
[4] Huayi Feng. Research on treatment and utilization methods of leachate in domestic waste landfill China Resources Comprehensive Utilization, 2021, 39 (06): 43 – 45
[5] Shengguang Fan. Characteristics analysis of metal ions and microbial community structure at different treatment stages of landfill leachate. Chang'an University, 2018.
[6] Lina Wu, Nannan Tu, Jikun Cheng. Research on water quality characteristics and treatment technology of landfill leachate. Science Technology and Engineering, 2014
[7] Hongxia Xiang. Research on antibacterial effect and electroanalytical technology for bioremediation and detection of pollutants. Hunan University. 2008, (09)
[8] Lin Liu. Screening and immobilization of low-temperature heterotrophic nitrifying bacteria Beijing University of Chemical Technology, 2020. DOI: 10. 26939/ d. cnki. gbhgu. 2020. 001389.
[9] Yonghui Su. Sewage collection, sampling and preservation in environmental monitoring. Leather Manufacture and Environmental Technology, 2021, 2 (12): 42 - 43.
[10] Konghuan Wu, Jufen Nie, Zhiyun Wang, Nengqin He. Screening and identification of denitrifying bacteria in constructed wetlands. Environmental Science Survey. 2014 (06): 6-9+38
[11] Shichang Liu, Yanqin Li, Fengchao Li, Xianjiang Kang. Isolation, screening and identification of a seawater ammonia nitrogen degrading bacterium Hebei Fisheries, 2017 (4): 11-13
[12] Xiaoling He, Danyu Zhao, Peilian Zhang, Screening of landfill leachate treatment strains and optimization of treatment process conditions. Journal of Tiangong University, 2019, 38 (05): 50 – 57
[13] Chunhua Zhang. Screening and identification of nitrate nitrogen transforming bacteria and study on their transformation efficiency. Shanghai Jiao Tong University. 2011 (07).
[14] Siyu Li, Xue Liu, Wenjing Wang. Microbial evolutionary tree construction method. Modern Agricultural Science and Technology, 2019, (19): 249 – 250 + 253
[15] Bei Wang. Research on phylogenetic tree construction and evaluation method based on dynamic multi-objective optimization. Northwest University, 2022.DOI:10.27405/d.cnki.gxbdu. 2022.001590.
[16] Shengmei Wu. Analysis of problems related to the spectrophotometric determination of ammonia nitrogen in water by Knott's reagent. Leather Manufacture and Environmental Technology, 2021, 2 Techniques (17): 7 – 8 + 12
[17] Xia Liu, Fengjiao Zeng, Hang Yu Determination of the number of acidophilus based on time, OD_ (600) value and plate counting. Gansu Medical Journal, 2020, 39 (09): 774 – 775 + 782. DOI: 10. 15975/ j. cnki. gsyy. 2020. 09. 003.
[18] Hongyan Zhang. Application of electrochemical combination process in industrial wastewater recycling. Jilin University, 2013.
[19] Lehua Zhang, Jian Guo. Discussion on the drawing and calculation formula of ammonia nitrogen, total nitrogen and total phosphorus calibration curves in water quality analysis Environment and Development, 2020, 32 (04): 228 - 229. DOI: 10. 16647/ j. cnki. cn15 - 1369/ X. 2020. 04. 132. 

Downloads: 2157
Visits: 121981

Sponsors, Associates, and Links

All published work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright © 2016 - 2031 Clausius Scientific Press Inc. All Rights Reserved.