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To Investigate the Molecular Mechanism of Wugedan Pill in Improving Insulin Resistance Based on Network Pharmacology

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DOI: 10.23977/medcm.2022.040305 | Downloads: 10 | Views: 580

Author(s)

Meng Zhang 1, Bo Lu 1

Affiliation(s)

1 Shaanxi University of Chinese Medicine, Xianyang, China

Corresponding Author

Bo Lu

ABSTRACT

Objective To explore the network pharmacological mechanism of Wugedan Pill in improving insulin resistance (IR). Methods The main active ingredients and corresponding target genes of Wugedan Pill were retrieved from the Traditional Chinese Medicine System Pharmacology Technology Platform (TCMSP), and the related targets of insulin resistance were obtained through the Human Gene Database (GeneCards) and the Human Online Mendelian Inheritance Database (OMIM). Gene, the active ingredient target of the drug is mapped with the insulin resistance target, and the intersection target is obtained, which is the predicted target of Wugedan Pill acting on insulin resistance. The "pharmaceutical active ingredient-target" network model was constructed by Cytoscape 3.7.0 software, and the key active ingredients were selected. The STRING database was used to construct an intersecting target-protein interaction network (PPI), and key proteins were selected. Intersection targets were subjected to Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis using the Metascape platform. Results There are 94 active ingredients and 163 related targets of Wugedan Pill acting on insulin resistance. The key active ingredients are quercetin, β-sitosterol and kaempferol, and the key proteins are estrogen receptor 1 (ESR1), aldose reductase (AR), nuclear receptor coactivator protein 1 (NCOA1), Fos protein et al.; GO enrichment analysis showed that Wugedan Pill could affect the activities of oxidoreductase, tyrosine kinase-binding protein, steroid-binding protein and other proteins in improving insulin resistance; KEGG pathway enrichment analysis showed that the effect was the most Notably lipid and atherosclerotic pathways, also significantly affected diabetes complications AGE-RAGE signaling pathway, PI3K-Akt signaling pathway, neuroactive ligand-receptor interactions, alcoholic liver disease, prostate cancer, cancer miRNAs expression, pancreatic cancer, thyroid hormone signaling and other pathways. Conclusion Wugedan Pill may play a role in improving insulin resistance, regulating lipid metabolism, reducing blood glucose and preventing complications by regulating the above targets and pathways with the effective ingredients quercetin, β-sitosterol and kaempferol.

KEYWORDS

Wugedan Pill, insulin resistance, network pharmacology, quercetin, kaempferol, β-sitosterol

CITE THIS PAPER

Meng Zhang, Bo Lu, To Investigate the Molecular Mechanism of Wugedan Pill in Improving Insulin Resistance Based on Network Pharmacology. MEDS Chinese Medicine (2022) Vol. 4: 31-39. DOI: http://dx.doi.org/10.23977/medcm.2022.040305.

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