Author(s)

Long Chen ¹

Affiliation(s)

¹ Nanjing Kucheng Chemical Technology Co., Ltd., National Science Park of Nanjing University of Science &Technology, 129-3 Guanghua Road, Nanjing, 210014, China

Corresponding Author

Long Chen

ABSTRACT

Four strains NKCT1101, NKCT1102, NKCT1103 and NKCT1104 isolated from wastewater of the washing workshop can utilize Isophthalonitrile (IPN) as the sole source of carbon and nitrogen. From the result of the physiological and biochemical experiments, the bacteria were similar to Sporosarcina, Micrococcus, Staphylococcus and Azotobacter respectively. In the study, several batch experiments were conducted to investigate the different degradation rate of the four strains, the biodegradation of IPN by NKCT1101. The result showed that degradation rate of IPN by NKCT1101 (about 90%) was much higher than that by NKCT1102, NKCT1103 and NKCT1104 (all about 70%). NKCT1101 could degrade IPN observably at the low biomasses. Even of different initial concentration, NKCT1101 could degrade IPN to a low residual concentration (10mg l-1). The additional carbon glucose and the additional nitrogen urea and Ammonium sulfate which not affect the degradation rate of NKCT1101. Finally NKCT1101 transformed IPN to corresponding acid by nitrilase. And the molecular weight of the nitrilase was 76kD.

KEYWORDS

isophthalonitrile, biodegradation, sporosarcina, nitrilase

CITE THIS PAPER

Long Chen. Biodegradation of Isophthalonitrile by Sporosarcina NKCT1101 Isolated from Wastewater of the Washing Workshop. Environment, Resource and Ecology Journal (2020) 4: 23-30. DOI: http://dx.doi.org/10.23977/erej.2020.040103.

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