Author(s)

Zhang Lei ¹, Yang Feng ², Zhu Junzhe ³

Affiliation(s)

¹ Zhejiang Gongshang University, Hangzhou, Zhejiang, 310018, China
² Community Health Service Center, Hangzhou, Zhejiang, 310000, China
³ Wenzhou Medical University, Wenzhou, Zhejiang, 310000, China

Corresponding Author

Zhang Lei

ABSTRACT

The CPV+CCV+Giardia Antigen Combo Rapid Test Cassette is a chromatographic immunoassay designed for the qualitative simultaneous detection of canine parvovirus (CPV) antigen, canine coronavirus (CCV) antigen and Giardia lamblia antigen in feces and vomit specimens from dogs. This study aimed to do the in-house clinical studies of CPV+CCV+Giardia Antigen Rapid Test Cassette (Feces/Vomit) with the CPV+CCV+Giardia positive specimen and CPV+CCV+Giardia negative specimen. A total of 423 specimens (209 feces and 214 vomit) were collected, including clinically confirmed positive and negative specimens for CPV, CCV and Giardia. The results demonstrated high relative sensitivity (CPV: 95.74%, CCV: 93.10%, Giardia: 95.12%), relative specificity (CPV: 100.00%, CCV: 97.52%, Giardia: 95.87%) and accuracy (CPV: 97.56%, CCV: 96.09%, Giardia: 95.68%) across all three targets, with narrow 95% confidence intervals (CIs) indicating robust reliability. The test exhibited consistent performance in both feces and vomit specimens, with results readable within 5-10 minutes, making it suitable for point-of-care settings. These findings indicate that the CPV+CCV+Giardia Antigen Combo Rapid Test Cassette is a reliable, rapid and practical tool for the simultaneous detection of multiple enteric pathogens in dogs, aiding in timely diagnosis and targeted treatment.

KEYWORDS

Canine Parvovirus (CPV); Canine Coronavirus (CCV); Giardia Lamblia; Antigen Combo Rapid Test; Feces; Vomit; Chromatographic Immunoassay; Veterinary Diagnostics

CITE THIS PAPER

Zhang Lei, Yang Feng, Zhu Junzhe, Diagnostic Efficacy of a Combined Antigen Rapid Test for CPV, CCV, and Giardia in Canine Feces and Vomit Specimens. MEDS Clinical Medicine (2025) Vol. 6: 27-31. DOI: http://dx.doi.org/10.23977/medsc.2025.060504.

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