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  4. Vol 5, Issue 5, 2024

Application of Decellularized Bone Matrix and Chitosan Composite Scaffold in Bone Defect Repair

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DOI: 10.23977/medsc.2024.050517 | Downloads: 8 | Views: 243

Author(s)

Lei Zhang 1, Xinhua Cheng 1

Affiliation(s)

1 Department of Orthopaedics, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China

Corresponding Author

Xinhua Cheng

ABSTRACT

Repair materials for bone defects often have problems such as poor biocompatibility, immune rejection and unsatisfactory healing effects. How to develop a scaffold material that has good biocompatibility and can effectively promote bone regeneration has become one of the hot topics in current research. This paper tests a composite scaffold of decellularized extracellular matrix (dECM) and chitosan to explore its application in bone defect repair. First, composite scaffolds of dECM and chitosan with different ratios are prepared to optimize the structure and properties of the material. Then, the micromorphology, mechanical properties and biocompatibility of the composite scaffolds are systematically evaluated using scanning electron microscope (SEM), mechanical properties test and in vitro cell culture experiments. In the repair experiment of the mouse bone defect model, the composite scaffold with a 1:1 ratio shows the highest bone density (0.45 g/cm²) at 8 weeks. The mechanical test results show that the composite scaffold with a 1:1 ratio is superior to other groups in terms of compressive strength, tensile strength and elastic modulus, and is close to the mechanical properties of natural bone. Immunohistochemical analysis show that the expression of bone formation markers such as ALP (alkaline phosphatase) and HE (Hematoxylin and Eosin) is significantly increased in the 1:1 composite scaffold group. In the above data, the dECM and chitosan 1:1 composite scaffold shows good biological activity, mechanical properties and repair effect in bone defect repair, and has great clinical application potential.

KEYWORDS

Bone Defect Repair; Acellular Bone Matrix; Chitosan; Composite Scaffold; Biomaterial

CITE THIS PAPER

Lei Zhang, Xinhua Cheng. Application of Decellularized Bone Matrix and Chitosan Composite Scaffold in Bone Defect Repair. MEDS Clinical Medicine (2024) Vol. 5: 119-127. DOI: http://dx.doi.org/10.23977/medsc.2024.050517.

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