Author(s)

Zixia Feng ¹

Affiliation(s)

¹ The Hong Kong University of Science and Technology, Hong Kong, 999077, China

Corresponding Author

Zixia Feng

ABSTRACT

The purpose of this paper is to discuss the application, advantages and disadvantages of Capillary Electrophoresis (CE) in the separation and detection of biological macromolecules, and to discuss the latest progress of this technology in the separation and detection of biological macromolecules. As a separation technology based on the migration velocity difference of ions or charged particles under the action of electric field, CE has been widely used in biochemistry, molecular biology, biomedicine and other fields. Firstly, this paper introduces the basic principle and development of CE, and emphasizes the importance of biomacromolecules research. Then, the application of CE in the separation and detection of biological macromolecules is described in detail, including the advantages of separation efficiency, analysis speed and sample consumption, as well as the limitations. Finally, the advantages and disadvantages of CE are summarized, and the future development direction and application prospect are put forward. Although CE has the advantages of high separation efficiency, rapid analysis and low sample consumption, it also has some limitations such as high requirements on sample properties. In the future, with the continuous improvement and development of technology, the application prospect of CE in the separation and detection of biological macromolecules will be broader.

KEYWORDS

Capillary Electrophoresis; Biological macromolecules; Separation and purification; Protein; Nucleic acid

CITE THIS PAPER

Zixia Feng, The Latest Development of Capillary Electrophoresis Technology in the Separation and Detection of Biological Macromolecules. Modern Physical Chemistry Research (2023) Vol. 3: 18-24. DOI: http://dx.doi.org/10.23977/mpcr.2023.030103.

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