Author(s)

Mingshan Pi, Xiaokang Gong, Mei Zhou, Qin Ru, Baomiao Ma, Changlong Leng, Xiang Tian and Yue Kai

Corresponding Author

Yue Kai

ABSTRACT

Purpose: To investigate the oxidative stress of diabetic mouse model established byhigh-fat diet combined with STZ. Methods: Twenty male C57 bl/6 mice were randomly divided into normal group and model group. Model group mice received a single intraperitoneal injection of streptozotocin(STZ) at a dose of 100 mg / kg after 4 weeks ofhigh-fat diet. Model group mice continue to take ahigh-fat diet for 1 week, then measure weight, fasting blood glucose (FPG), and take samples to measure total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), Malondialdehyde(MDA)and Superoxide dismutase(SOD) in serum; MDA, SOD, glutathione peroxidase(GSH-Px) and catalase(CAT) in tissues ofheart, liver, kidney, pancreas,hippocampus and cortex. Results: Compared with the normal group, FPG, TC, TG and LDL-C in the diabetic mice were significantly increased (P<0.05). In serum, the SOD activity of the diabetic mice was significantly lower than that of the normal group (P<0.05); the MDA content was significantlyhigher than that of the normal group (P<0.05). The MDA content in theheart tissues of mice in the diabetic group was significantlyhigher than that in the normal group (P<0.05); the CAT activity was significantly lower than that in the normal group (P<0.05). MDA content in liver tissues of mice in the diabetic group was significantlyhigher than that in the normal group (P<0.05); SOD and GSH-Px activities were significantly lower than those in the normal group (P<0.05). MDA content in kidney tissues of mice in diabetes group was significantlyhigher than that in normal group (P<0.05). The MDA content and GSH-Px activity in pancreatic tissue of mice in the diabetic group were significantlyhigher than those in the normal group (P<0.05); SOD and CAT activities were significantly lower than those in the normal group (P<0.05). Conclusion: Diabetic mice induced byhigh fat diet combined with STZ showed obvious tissue oxidative stress.

KEYWORDS

Diabetes, mice, tissue, oxidative stress