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Anti-TMVActivity of Atisine-type Diterpene Alkaloids in Spiraea Japonica Associates with Down-Regulating the Expression of TMV Coat Protein

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DOI: 10.23977/trebe.2020.11002 | Downloads: 16 | Views: 1904

Author(s)

Xinying Mao 1,2, Chunlin Wang 1,2, Wuling Liu 1,2, Jia Yu 1,2, Zekun Sun 2, Mengling Wang 2, Heng Luo 1,2

Affiliation(s)

1 State key laboratory of functions and applications of medicinal plants, Guizhou medical university, Guiyang, People’s Republic of China
2 The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550014, People's Republic of China

Corresponding Author

Xinying Mao

ABSTRACT

Four atisine-type diterpene alkaloids, including hsp-X40, hsp-X35, hsp-X31 and hsp-X26, were derivatives of diterpenes isolated from the ethanol extract of Spiraea japonica and then their chemical structures were determined with spectroscopy techniques. The result indicated that four compounds at a concentration of 100µg/mL can inhibit the infection of TMV to tobacco through in vivo curative activity, with the curative rate of 55.4±1.5% of hsp-X26, 53.2±1.9% of hsp-X31, 58.1± 2.0% of hsp-X35, 60.3±1.2% of hsp-X40, which was lower (p< 0.05) than that of the positive control Ningnanmycin (55.2±1.9%). However, the four compounds have not shown a better protective effect for tobacco against the TMV infection. The compound hsp-x40 with the diterpene amino acid ester group exhibited its IC50 value was 76.7 ± 2.4 μg/mL, suggesting that diterpene amino acid ester group may be as the important functional group for the anti-TMV activity of atisine-type diterpene alkaloids. The molecular experimental results showed that four active compounds can down-regulate the expression of TMV coat protein, indicating that the anti-TMV activity of atisine-type diterpene alkaloids in spiraea japonica associates with down-regulating the expression of TMV coat protein.

KEYWORDS

Atisine-type Diterpene alkaloids, Tobacco mosaic virus (TMV), Coat protein, Curative activity

CITE THIS PAPER

Xinying Mao, Chunlin Wang, Wuling Liu, Jia Yu, Zekun Sun, Mengling Wang, Heng Luo, Anti-TMVActivity of Atisine-type Diterpene Alkaloids in Spiraea Japonica Associates with Down-Regulating the Expression of TMV Coat Protein. Trends in Biochemical Engineering (2020) 1: 20-29. DOI: http://dx.doi.org/10.23977/trebe.2020.11002.

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