Anti-TMVActivity of Atisine-type Diterpene Alkaloids in Spiraea Japonica Associates with Down-Regulating the Expression of TMV Coat Protein
DOI: 10.23977/trebe.2020.11002 | Downloads: 16 | Views: 1904
Xinying Mao 1,2, Chunlin Wang 1,2, Wuling Liu 1,2, Jia Yu 1,2, Zekun Sun 2, Mengling Wang 2, Heng Luo 1,2
1 State key laboratory of functions and applications of medicinal plants, Guizhou medical university, Guiyang, People’s Republic of China
2 The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550014, People's Republic of China
Corresponding AuthorXinying Mao
Four atisine-type diterpene alkaloids, including hsp-X40, hsp-X35, hsp-X31 and hsp-X26, were derivatives of diterpenes isolated from the ethanol extract of Spiraea japonica and then their chemical structures were determined with spectroscopy techniques. The result indicated that four compounds at a concentration of 100µg/mL can inhibit the infection of TMV to tobacco through in vivo curative activity, with the curative rate of 55.4±1.5% of hsp-X26, 53.2±1.9% of hsp-X31, 58.1± 2.0% of hsp-X35, 60.3±1.2% of hsp-X40, which was lower (p< 0.05) than that of the positive control Ningnanmycin (55.2±1.9%). However, the four compounds have not shown a better protective effect for tobacco against the TMV infection. The compound hsp-x40 with the diterpene amino acid ester group exhibited its IC50 value was 76.7 ± 2.4 μg/mL, suggesting that diterpene amino acid ester group may be as the important functional group for the anti-TMV activity of atisine-type diterpene alkaloids. The molecular experimental results showed that four active compounds can down-regulate the expression of TMV coat protein, indicating that the anti-TMV activity of atisine-type diterpene alkaloids in spiraea japonica associates with down-regulating the expression of TMV coat protein.
KEYWORDSAtisine-type Diterpene alkaloids, Tobacco mosaic virus (TMV), Coat protein, Curative activity
CITE THIS PAPER
Xinying Mao, Chunlin Wang, Wuling Liu, Jia Yu, Zekun Sun, Mengling Wang, Heng Luo, Anti-TMVActivity of Atisine-type Diterpene Alkaloids in Spiraea Japonica Associates with Down-Regulating the Expression of TMV Coat Protein. Trends in Biochemical Engineering (2020) 1: 20-29. DOI: http://dx.doi.org/10.23977/trebe.2020.11002.
 BOS L. Crop Losses Caused by Viruses. Crop Protection. 1982; 1(3): 263-270.
 Wang C-M, Su H, Hao C, et al. Preliminary study on the mechanism of anti-TMV effects of natural compound eugenol. Research and Development. 2012; (51): 29-31.
 Ouyang M, Wein Y-S, Zhang Z-K, et al.Inhibitory activity against tobacco mosaic virus (TMV) replication of pinoresinol and syringaresinol lignans and their glycosides from the root of Rhusjavanica var. Roxburghian .Agric Food Chem. 2007; 55(16): 6460-6465.
 Wu Z-J, Ouyang M, Wang C-Z, et al.Anti-tobaccomosaic virus (TMV) triterpenoid saponins from the leaves of Ilex oblonga. Agric Food Chem. 2007; 55 (5): 1712 - 1717.
 Shen J-G, Zhang Z-K, Wu Z-J,et al. Antiphytoviral activity of bruceine-D from Brucea javanica seeds. Pest Manage Science. 2008; 64 (2): 191-196.
 Yao Y-C, An T-Y, Gao J,et al. Research of chemistry and bioactivity of active compounds antiphytovirus in Cynanehum komarovii. Chin J Org Chem (Organic Chemistry). 2001; 21 (11): 1024-1028.
 Chen Q-J, Ouyang MA ,Xie L-H, et al. Isolation and detection of anti-TMV activity constituent from Parthenium hysterophorus. Acta Laser Biol Sin (Laser Bio Journal). 2008; 17 (4): 544-548.
 LI Y-M, WANG L-H, LI S-L, et al. Seco-pregnaneSteroids Target the Subgenomic RNA of Alphavirus-like RNA Viruses. Proceedings of the National Academy of Sciences. 2007; 104 (19): 8083-8088.
 Zhu L, Lin X-M, Zhong M, et al. Preliminary study on the inhibitory activity of Pleurotus ostreatus protein extract on tobacco mosaic virus. Modern Agricultural Science and Technology. 2009; 12: 12-14.
 Lin S-W, He Y-X,Screening of plant extracts against tobacco mosaic virus activity. Anhui Agricultural Science Bulletin. 2009; 15 (9): 146-148.
 Zhai M-Z, Li P-Y, Huang H-L, Effect of walnut green bark active substance P4-2 on physiological and biochemical indexes in tobacco. Journal of Northwest Forestry University. 2009; 24 (4): 143-146.
 Ma-Y, Mao X-Y , Huang L-J,et al. Diterpene alkaloids and diterpenes from Spiraea japonica and their anti-tobacco mosaic virus activity. Fitoterapia, 2016; 109: 8-13.
 Yang D-Q, Chen Z-R, Chen D-Z, et al. Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata and Lycoricidine Derivatives. Natural Products and Bioprospecting, 2018; 5, (8): 189-197.
 Wu Y-X, Inhibition of TMV by triadimefon and induction of tobacco resistance. Hebei Agricultural University 20080601.
Li H-X, The inhibitory effect of several antiviral agents on tobacco mosaic virus and related mechanisms. Hebei Agricultural University 20100601.
 Wu Y-B, Xie L-Y, Xie L-H, et al. Effect of Polysaccharide CCP60a on the coat protein of TMV. Journal of Plant Resources and Environment. 2008; 17 (3): 63-66.