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Molecular Screening and Identification of Lactobacillus acidophilus Producing β-Galactosidase

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DOI: 10.23977/enzyme.2022.010101 | Downloads: 8 | Views: 471

Author(s)

Oparaji Emeka H 1

Affiliation(s)

1 Department of Chemical Sciences, Spiritan University, Nneochi, Abia State

Corresponding Author

Oparaji Emeka H

ABSTRACT

β-galactosidase producing Lactobacillus was isolated from dairy industrial waste water collected from Rumuekini, Rivers state. Physicochemical properties of the diary effluent showed the following: pH (5.5); Conductivity (845 Ω-1cm-1); Dissolved oxygen (6.24 mg/ml); Biochemical oxygen demand (5.22 mg/ml), Total oxidizable carbon contents (72.6 mg/ml), Total organic matter content (89.30 mg/ml) and Temperature (37.67°C).  Total solids, suspended solid and total dissolved solids of the waste water were: 618.84, 82.65 and 536.19 mg/ml, respectively. The basic morphological features of the bacteria showed that the isolate are: rod shaped (Bacilli), non sporulating and non motile bacteria; biochemically, they are obligate gram-positive, starch hydrolyzing and lactic acid forming organisms with optimum growth at temperature of 25-40oC. Electrophoretogram of the amplified genome of Lactobacilli showed a typical band at 750 bp. Lactobacillus acidophilus was identified after the genomic sequencing with ascribed NCBI accession number of JX255677. p-NPG and o-NPG used in the nutrient broth in the presence of the bacteria showed positive test for β-galactosidase production with yellow colouration after three days of incubation, however the colouration was found intense in broth infused with p-NPG. Optimum bacteria growth was observed at 36th hour of incubation with 2ml of the microbial suspension containing the highest heterotrophic counts of the organism (5.4 x 108 CFU/ml). Highest β-galactosidase activity and protein concentration were obtained on day 12 (126µmol/min) and day 13 (0.72 mg/ml) of the incubation at pH 6.0.

KEYWORDS

β-galactosidase, p-NPG, o-NPG, Lactobacillus acidophilus, physicochemical

CITE THIS PAPER

Oparaji Emeka H, Molecular Screening and Identification of Lactobacillus acidophilus Producing β-Galactosidase. Journal of Enzyme Engineering (2022) Vol. 1: 1-15. DOI: http://dx.doi.org/10.23977/enzyme.2022.010101.

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