Author(s)

Liqiong Wang ¹

Affiliation(s)

¹ Department of Medicine, Changde Vocational and Technical College, Changde, China

Corresponding Author

Liqiong Wang

ABSTRACT

Objective to explore the influence of smoking on respiratory microbiota. Methods the traditional bacterial culture method was used to analyze the bacterial colonies qualitatively and quantitatively. The 16SrDNA fragment of characteristic bacterial colonies was amplified by PCR and sequenced, and then compared with the NCBI gene sequence library to analyze the changes of bacterial communities in the secretion of the pharyngeal wall of healthy smokers and non-smokers. Results the bacteria detection rate of smokers was significantly higher than that of non-smokers (P < 0. 05). Conclusion Smoking can increase the amount of bacteria in oropharynx and increase the incidence of pathogenic bacteria, so that the respiratory tract flora imbalance.

KEYWORDS

Smoking, respiratory flora, dysbacteriosis

CITE THIS PAPER

Liqiong Wang. Effect of Smoking on Respiratory Flora. Bacteriology and Microbiology (2021) Vol. 1: 1-4. DOI: http://dx.doi.org/10.23977/bactm.2021.010101.

REFERENCES

[1] Wang, C., Liu, J.Y. (2015) Research progress on the role of cigarette smoke in the pathogenesis of chronic obstructive pulmonary disease. Journal of Applied Medicine, 31(1), 8-9.
[2] Dang, R.Y, Zhao, C.Y, Fan, X, et al. (2018) Effects of cigarette smoke exposure on the distribution of bacteria in the lower respiratory tract of normal mice. Shandong Medicine, 58(20), 9-12.
[3] Pu, J.D., Huang, L.M., Ye, X.Q., et al. (2016) Effects of fine particulate matter in the environment on transdifferentiation of human bronchial epithelial cells. Chinese Journal of Tuberculosis and Respiratory Diseases, 39(10), 784-790.
[4] Simon, C., Daniel, R. (2011) Metagenomic analyses: past and future trends. Appl Environ Microbiol, 77(4), 1153-1161.
[5] Singh, B.K., Macdonald, C.A. (2010) Drug discovery from uncultivable microorganisms. Drug Discov Today, 15(78), 792-799.

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