Author(s)

Ying Meng ^1,2, Yufeng Zhang ², Linlin Chen ¹, Weihong Gao ¹, Danni Wang ¹, Xin Chen ¹

Affiliation(s)

¹ School of Management, Liaoning University of International Business and Economics, Dalian, Liaoning, China
² School of Management, Department of Computing, Rattana Bundit University, Bangkok, Thailand

Corresponding Author

Ying Meng

ABSTRACT

A HPLC method was established for the determination of 10 α - hydroxy acids, glucuronic acid, tartaric acid, glycolic acid, malic acid, l actic acid, citric acid, 2-hydroxybutyric acid, mandelic acid, diphenylethanoic acid and hydroxyoctanoic acid in cosmetics. The sample was separated on the CAPCELL PAK C18 MG column(4.6×250,5μm),with 0.1 mol / L diammonium hydrogen phosphate solution as mobile phase. The flow rate was 1 mL•min-1, and the column temperature was 25 ℃. Linearity of glucuronic acid, tartaric acid, glycolic acid, malic acid, lactic acid, citric acid,2-hydroxybutyric acid, mandelic acid, diphenylethanoic acid and hydroxyoctanoic acid standards were established within the range of 103.5~2070 μg•mL-1,65~1300 μg•mL-1, 101.5~2025 μg•mL-1, 113.35~2025 μg•mL-1, 113.35~2267 μg•mL-1, 250~5000 μg•mL-1, 105~2100 μg•mL-1, 126.35~2527 μg•mL-1, 2.96~59.2 μg•mL-1,3.1~62 μg•mL-1, and 105.75~2115 μg•mL-1 with the correlation coefficient above 0.997 respectively. The average recoveries were 90.46%-96.48%,90.13%- 100.16%, 92.81%-98.73%,92.84%-97.74%,94.11%-100.17%,86.73-115.12,90.13-98.99,87.04-96.73,90.23-95.41,92.54-96.55and91.76-100.44 ,respectively. Tartaric acid and malic acid were not detected in four samples. The contents of hydroxyacetic acid were 3.31%-10.02%. The content of lactic acid was 1.51% in one sample. The content of citric acid was 0.03% in one sample. In 10 batches of samples, α - hydroxy acid, glucuronic acid, tartaric acid, glycolic acid, malic acid, mandelic acid and diphenyl alcohol acid were not detected. The content of lactic acid S11 was 1.57%, citric acid S1 and S2 were 0.22% and 0.17%, 2-hydroxybutyric acid S1 and S8 were 2.71% and 1.47%, and hydroxyoctanoic acid S11 was 0.12%. All 12 kinds of cosmetics were qualified and did not exceed the national content requirements. The method is simple, sensitive and reproducible, and can be used for the determination of α - hydroxy acid in cosmetics.

KEYWORDS

Cosmetics; Alpha-hydroxy acids; Glucuronic acid; Citric acid; 2-hydroxybutyric acid

CITE THIS PAPER

Ying Meng, Yufeng Zhang, Linlin Chen, Weihong Gao, Danni Wang, Xin Chen, Determination of the Content of α-Hydroxy Acids in Cosmetics by HPLC. Analytical Chemistry: A Journal (2024) Vol. 3: 46-53. DOI: http://dx.doi.org/10.23977/analc.2024.030109.

REFERENCES

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[3] Liang Xue. Application and Research Progress of Plant Extracts in Anti-Aging Cosmetics. [J]. Journal of Taiyuan University of Technology. 2009, (10): 66-67.
[4] Hygienic Standard for Cosmetics [S]. 2007: 248.
[5] Sun Jun, Shen Lu, Li Hao, et al. Overview of Cosmetic Safety Evaluation in the United States and Its Implications. China Pharmacy [J]. 2015, 26(19): 2604-2607. 
[6] Lu Chun, Yu Xiaoping, Huang Guangrong, et al. Simultaneous Detection of Six Hydroxy Acids in Cosmetics by High-Performance Liquid Chromatography. Daily Chemical Industry [J]. 2009, 39(05): 361-364.
[7] Sun Wenshan, Tian Furui, Wang Xuqing, et al. Determination of Five α-Hydroxy Acids in Cosmetics by High-Performance Liquid Chromatography. Fragrance & Flavor Cosmetics [J]. 2012, (05): 25-28.

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