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Effect of specimen placement time and temperature on RBC detection in urine

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DOI: 10.23977/analc.2024.030105 | Downloads: 8 | Views: 230

Author(s)

Chengbi Tong 1, Liu Peng 2, Yaxing Zhu 1, Tianhong Jia 1

Affiliation(s)

1 Clinical Laboratory Department, Affiliated Hospital of Hebei University, Baoding, China
2 Baoding Maternal and Child Health Care Hospital, Baoding, China

Corresponding Author

Tianhong Jia

ABSTRACT

The accuracy of red blood cell count and morphology in urine samples is of great value for clinical diagnosis. In this study, a total of 215 urine samples were collected from affiliated Hospital of Hebei University from October 2022 to April 2023, among which 112 cases were placed at room temperature, 103 cases in 4℃ refrigerator, UF-5000 urine formed analyzer measured their placement after 0.5h, 1h, 2h, 4h, 6h, observed the changes of red blood cell morphology at each time point and analyzed the significant difference with 0.5h by t-test. At room temperature, red blood cells in urine at 2h, 4h and 6h were significantly compared with 0.5h (P <0.05), and at 1h and 0.5h were not significant (P> 0.05). In 4℃ environment, the results in urine at 4h and 6h were compared with those at 0.5h (P <0.05), and at 1h, 2h and 0.5h were not significant (P> 0.05). Therefore, urine samples at room temperature should be completed before 2h, preferably not more than 1h. Under 4℃ refrigerator storage conditions, the test can be completed before 4h, preferably no more than 2h.

KEYWORDS

Urine; red blood cell count; morphology; time; temperature

CITE THIS PAPER

Chengbi Tong, Liu Peng, Yaxing Zhu, Tianhong Jia, Effect of specimen placement time and temperature on RBC detection in urine. Analytical Chemistry: A Journal (2024) Vol. 3: 26-29. DOI: http://dx.doi.org/10.23977/analc.2024.030105.

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