Author(s)

Tianxing Shi ¹, Jinyan Xue ¹, Yueying Wang ¹, Yulu Chang ¹, Yuzi Wang ¹, Liman Liang ¹, Kui Niu ¹

Affiliation(s)

¹ School of Chemical Engineering, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, 066004, China

Corresponding Author

Kui Niu

ABSTRACT

Chestnuts, characterized by their dual attributes of nutritional and health-promoting properties, are notably rich in polysaccharide constituents. In this study, we employed hot water extraction to extract the water-soluble saccharide components from chestnut kernels. The extracted solution underwent membrane separation and column chromatography for the isolation and purification of polysaccharides, yielding N-CP, a neutral polysaccharide with a purity of 95.20%. N-CP exhibited characteristic infrared absorption features of typical polysaccharide compounds, consisted primarily of arabinose, galactose, and glucose, with a weight-average molecular weight of 482.34 kDa. The anti-proliferative activity of N-CP was evaluated against Hela and A549 cancer cell lines using the CCK-8 assay, with respective IC50 values of 35.45 µg/mL and 25.94 µg/mL. The results offer an efficient method for the extraction and purification of functional chestnut polysaccharides, laying a foundation for their application in functional foods and anti-tumor pharmaceuticals.

KEYWORDS

Physicochemical property, Antitumor activity, Chestnut polysaccharide, Membrane filtration

CITE THIS PAPER

Tianxing Shi, Jinyan Xue, Yueying Wang, Yulu Chang, Yuzi Wang, Liman Liang, Kui Niu, Physicochemical Properties and Antitumor Activities of Polysaccharides Separated from Chinese Chestnut by Membrane Filtration. Advances in Food Science and Human Nutrition (2023) Vol.5: 69-76. DOI: http://dx.doi.org/10.23977/afshn.2023.050109.

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