Author(s)

Tao Dong ¹, Chan Jiao ², Yuanjing Qiu ¹, Chao Han ¹

Affiliation(s)

¹ Kaifeng Product Quality Inspection and Testing Center, Kaifeng, Henan, 475000, China
² Kaifeng University, Kaifeng, Henan, 475000, China

Corresponding Author

Tao Dong

ABSTRACT

In accordance with the steps of the second method of GB 4789.10-2016, culture experiments were carried out on standard strains of Staphylococcus aureus, isolates from food samples using S. aureus chromogenic medium and Baird-Parker medium. There was no difference between the quantitative detection results of the two media in the concentration of S. aureus from 20 to 200 CFU/mL (P>0.05), the colonies on the Baird-Parker plate were smaller, black and without turbid band when cultured for 24 h. The characteristics of typical colonies were not obvious, and typical colonies appeared when the culture was prolonged to 48 h. The colonies appeared with typical characteristics of growth, black and turbid band; the colonies showed pinkish-purple when cultured for 18 h on the colour-developing plate, and the detection efficiency of colour-developing plate was superior to that of Baird-Parker plate. When cultured for 18 h on the chromogenic plate, the colonies were pinkish-purple, and the detection efficiency of the chromogenic plate was better than that of the Baird-Parker plate, and the incubation time could be shortened by about 24h. The quantitative detection effect of the chromogenic medium and the Baird-Parker medium on Staphylococcus aureus was comparable, but the chromogenic medium could shorten the cultivation time and had a higher efficiency, and the sensitivity and specificity of chromogenic medium for the detection of the strains preserved in the laboratory were higher than that of the Baird-Parker medium. The sensitivity and specificity of the colour-developing medium were higher than those of the Baird-Parker medium.

KEYWORDS

Culture medium; Staphylococcus aureus; quantitative detection

CITE THIS PAPER

Tao Dong, Chan Jiao, Yuanjing Qiu, Chao Han, Comparison of Different Media for the Quantitative Detection of Staphylococcus Aureus. Advances in Food Science and Human Nutrition (2024) Vol.6: 1-6. DOI: http://dx.doi.org/10.23977/afshn.2024.060101.

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