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Isolation and Identification of Aquamicrobium Strains from a Fecal Contaminated Sludge Sample

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DOI: 10.23977/phpm.2021.010103 | Downloads: 11 | Views: 1457

Author(s)

Chengru Wu 1, Chunyan le 1, Tianwen Gao 1, Yingsong Zheng 2, Jinjun Ji 3

Affiliation(s)

1 College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
2 The First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
3 College of Basic Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China

Corresponding Author

Jinjun Ji

ABSTRACT

The metabolism of cholesterol by bacteria may impact human health directly and indirectly. To elucidating the degradation process of cholesterol is of great benefit to understand the relationship between environmental microbes and human health. Isolation and identification cholesterol-degrading microorganisms is a basic work to obtaining superior experimental materials. Serial dilution agar plating and agar plate streaking were used to obtain single and pure bacterial colony. Sanger sequencing, Gram staining, and some physiological and biochemical reaction assays were used to characterize isolated bacteria. Two strains, Aqu1 and Aqu2, were isolated and purified from a fecal contaminated sludge sample. The colonies of Aqu1 and Aqu2 were variety of milky white-color. Both strains were gram negative, spherical shaped Aquamicrobium genus bacteria. The 16s rDNA of Aqu1 and Aqu2 was highly identity to that of A. aestuarii and A. lusatiense, respectively.

KEYWORDS

Cholesterol degradation, Bacterial isolation, Biochemical classification, Physiological classification, 16s rdna taxonomic study

CITE THIS PAPER

Chengru Wu, Chunyan le, Tianwen Gao, Yingsong Zheng, Jinjun Ji, Isolation and Identification of Aquamicrobium Strains from a Fecal Contaminated Sludge Sample. MEDS Public Health and Preventive Medicine (2021) 1: 18-22. DOI: http://dx.doi.org/10.23977/phpm.2021.010103

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