Research Status of Antigen Synthesis Methods
DOI: 10.23977/jfsst.2021.010910 | Downloads: 4 | Views: 138
Yixin Ji 1,2, Yibo Zhang 3, Haihong Xin 1,2, Tingxin Wang 1
1 College of Quality and Technical Supervision, Hebei University, Baoding, China
2 Institute of Quality and Performance Management, Hebei University, Baoding, China
3 College of stomatology, Hebei Medical University, Shijiazhuang, China
Corresponding AuthorTingxin Wang
Most haptens are small molecules compounds. Because of its small molecular mass itself alone can not induce an immune response, it is necessary to form combined with the carrier hapten-carrier protein conjugate can stimulate the body's immune response. In recent years, in-depth research has been conducted at home and abroad on the synthesis of artificial antigens and the analysis of the results. Artificial antigen synthesis technology has been applied to many fields such as food and environment, which have more or less impact on our lives. This article reviews how to select carrier protein and the coupling method of hapten and carrier in the process of artificial antigen synthesis.
KEYWORDSHapten, Carrier protein, Artificial antigen, Coupling
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Yixin Ji, Yibo Zhang, Haihong Xin and Tingxin Wang. Research Status of Antigen Synthesis Methods. Journal of Frontiers of Society, Science and Technology (2021) 1: 68-75. DOI: http://dx.doi.org/10.23977/jfsst.2021.010910
 S Hatanaka; I Imamura; Y Terazawa; K Kuroda; G Endo. The Characteristics of Specific Igg to Phthalic Anhydride (Pa)-Albumin Conjugate[J]. Industrial health. 1997, Vol.35(No.4): 508-514.
 Wang, Jia; Bever, Candace R. S.; Majkova, Zuzana.Heterologous Antigen Selection of Camelid Heavy Chain Single Domain Antibodies against Tetrabromobisphenol A [J]. Analytical Chemistry. 2014, Vol.86(No.16): 8296-8302.
 Zheng Wei. Preparation and Identification of Ractopamine Artificial Antigen [D]. Zhengzhou University, 2014.
 Shi Rongfeng, Liu Wenping, Wang Xueqian, Zhao Yan, Qu Huihua, Zhao Yan, Wang Qingguo. Synthesis And Identification of Aloin Artificial Antigen [J]. Journal of Pharmaceutical Analysis, 2016, 36(03): 406-410.
 Mercader Josep V, Esteve-Turrillas Francesc A, Agulló Consuelo, Abad-Somovilla Antonio, Abad-Fuentes Antonio. Antibody Generation and Immunoassay Development in Diverse Formats for Pyrimethanil Specific and Sensitive Analysis [J]. The Analyst, 2012, 137(23).
 Sheng Wei, Xia Xunfeng, Wei Keyi, et al. Determination of Marbofloxacin Residues in Beef and Pork with an Enzyme-Linked Immunosorbent Assay. Journal of Agricultural and Food Chemistry, 2009, 57(13): 5971-5.
 .Simple and Sensitive Determination of Sparfloxacin in Pharmaceuticals and Biological Samples by Immunoassay [J].Journal of Pharmaceutical Analysis, 2012, 2(03): 214-219.
 Yuan Qiang, Liang Feimin, Wu Lixian. Synthesis and Identification of Zolpidem Antigen [J]. Chinese Journal of Drug Abuse Prevention and Treatment, 2021, 27(03): 289-293.
 Guangdong Science and Technology Association; Guangdong Science and Technology Committee; Zhou Zhenhong; Liang Xiang. The 20th Century Guangdong Science and Technology Records [M]. Guangzhou: Guangdong Economic Publishing House. 1998.
 Liu Jinping. Preparation of Doxycycline Monoclonal Antibody and Establishment of Elisa Detection Method [D]. Yangzhou University, 2016.
 Zhu, H.P.; Liu, T.T.; Liu, B.; Yin, H.L.; Li, X.L.; Wang, L.; Wang, S.. Antigens Synthesis and Antibodies Preparation for Furazolidone and its Metabolite 3-Amino-2-Oxazolidinone (Article) [J].Chinese Chemical Letters. 2010, Vol.21(No.9): 1049-1052.
 Hong Xiaozhuang, Sun Manji. Protein connection technology [M]. Beijing: China Medical Science and Technology Press, 1993: 2-3
 Li Chunsheng, Li Junhua, Wu Xiaoli, Li Suhong, Yan Jinghui, Wu Meng. Comparison of Cimaterol Immune Antigen Synthesis Methods[J]. Animal Husbandry and Veterinary Medicine. 2014, 46(8): 61-64.
 Ye Shengqiang. Preparation of Tylosin and Tilmicosin Monoclonal Antibody and Study on ELISA Test Kit [D]. Huazhong Agricultural University, 2008.
 Fang Zhenxia. Study on the Separation and Purification of Aflatoxin B_1 (AFB_1) and the Efficient Synthesis of Afb_1 Artificial Antigen [D]. Fujian Agriculture and Forestry University, 2014.
 Martin C N, Garner R C. Aflatoxins b-Oxide Generated by Chemical or Enzymic Oxidation of Aflatoxin b1 Causes Guanine Substitution In Nucleic Acids [J]. Nature, 1977, 267(5614); 863-865