Education, Science, Technology, Innovation and Life
Open Access
Sign In

Determination and Analysis of Promazine Hydrochloride in Chicken by High Performance Liquid

Download as PDF

DOI: 10.23977/jfsst.2021.010909 | Downloads: 7 | Views: 125


XiaoLi Han 1,2, YiQing Li 1,2, JiaYan Zheng 1,2, TingXin Wang 1,2


1 College of Quality and Technical Supervision, HeBei University, Baoding, China
2 Institute of Food Safety and Standardization, Hebei University, Baoding, China

Corresponding Author

TingXin Wang


To establish a method for the determination of promazine hydrochloride residue in pork by HPLC. The extraction of promazine hydrochloride from pork tissues by ultrasonic extraction and centrifugal extraction was studied. The peak area of promazine hydrochloride was determined by high performance liquid chromatography. The content of promazine hydrochloride in pork was calculated by standard curve equation. The mobile phase was acetonitrile-ammonium acetate (0.02mol/mL) with a ratio of 60:40, the detection wavelength was set at 258 nm, the flow rate was 1.0mL/min, and the sample size was 10μL. The column temperature was 20℃. Acetonitrile was selected as the extraction liquid, extracted by ultrasonic shock method and centrifugal method, purified by solid phase extraction method, and dried and concentrated by vacuum as the pre-treatment method of pork. The results showed that promazine hydrochloride showed a good linear relationship with the peak area in the concentration range of 1ug/mL~50ug/mL (r2=0.9983). The intra-day and intra-day precision were all less than 3%. The recoveries of three different concentrations were 79.31%~84.38%. The method used in this experiment is simple, easy to operate, high accuracy and good reproducibility, which is suitable for the analysis and detection of the residual amount of promazine hydrochloride in pork. 


Pork, Promazine hydrochloride, High pressure liquid chromatography, A sedative


XiaoLi Han, YiQing Li, JiaYan Zheng and TingXin Wang. Determination and Analysis of Promazine Hydrochloride in Chicken by High Performance Liquid. Journal of Frontiers of Society, Science and Technology (2021) 1: 59-67. DOI:


[1] Na,G.Q.( 2020) Research on Rapid Immune Detection Methods of Peptide Antibiotics, Phenothiazines and New "Clenbuterol" and Other Veterinary Drugs[D]. Jiangnan University.
[2] Wu,N.P., Ban,F.G., Peng Li., et al.(2012)Screening of 11 Drugs in Feed by Ultra Performance Liquid Chromatography-tandem Quadrupole Time-of-flight Mass Spectrometry [J]. Chinese Journal of Mass Spectrometry, 3, 94-98+117.
[3] Hou ,M.L., Dong,X.B., Li Hong, H.L.(2020) DrugResidues in Livestock and Poultry Meat by UPLC-MS/MS [J]. Food and Fermentation Science and Technology, 3, 113-117+126.
[4] Zhang,H., He X.M. (2019)Determination of Seven Sedatives in Feed by Improved QuEChERS Combined with High Performance Liquid Chromatography-tandem Mass Spectrometry [J]. Chinese Feed, 19, 83-87.
[5] Luo,H.Y., Xu P., Yan W.W., Chen.J.H.( 2017) Rapid Detection of Sedatives and Estrogen Residues in Pig Blood Tofu by Improved MSPD-HPLC [J]. Food Research and Development, 38, 147-150.
[6] Ke,H.K., Lv,S.N., Hao,H.X., Cai,N,B.(2020)Progress in The Detection Methods of Benzodiazepines [J]. Chinese Journal of Analytical Laboratory,39,1110-1116.
[7] Wang,l.p., Li x., Sun y., Zhao,h.x., Qiu,y.m., Zhong,w.k., Tang,y.z., Wang,d.n., Zhou,z.q. (2005) Determination of Benzodiazepine Residues in Pork by Gas Chromatography/Mass Spectrometry [J]. Chinese Journal of Analytical Chemistry,7, 951-954
[8] Zhang,l.q., Wu,p.g., Jin,q., Zhang,l.m., Wang,x.f., Ren,n.(2013).Progress in Chromatographic Detection of Sedative Residues [J]. Chinese Journal of Health Laboratory Technology, 6,1634-1638.
[9] Yang,T., Sun,Z.X., Bao,W.H. et al. (2010)Study on The Correlation Between The Elimination of Clenbuterol Residues in Pig Urine and Blood [D].Agricultural Science and Technology of Ningbo,8, 160-163.
[10] Cai,Y.G., Wu,Y.F., Wang,W., Zhang,L., Chang,J.(2020)Chinese Journal of Forensic Medicine, 4,411-413.
[11] Soad,S., Abd,El-Hay., Hisham,H. (2016).High Performance Liquid Chromatography for Simultaneous Determination of Xipamide,Triamterene and Hydrochlorothiazide in Bulk Drug Samples and Dosage Forms[J]. Acta Pharmaceutica, 109-118.
[12] Wang,Q. (2018) Application and Prospect of High Pressure Liquid Chromatography [J]. Food Safety Guide, 27, 186.

Downloads: 1013
Visits: 38853

All published work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright © 2016 - 2031 Clausius Scientific Press Inc. All Rights Reserved.